|
KMID : 0545119990090050646
|
|
Journal of Microbiology and Biotechnology
1999 Volume.9 No. 5 p.646 ~ p.649
|
|
|
Discovery of D-Stereospecific Dipeptidase from Thermophilic Bacillus sp.BCS-1 and Its Application for Synthesis of D-Amino Acid-Containing Peptide
|
|
BAEK, HAE-HEOUN
KWON, SEOK-JOON/PARK, JIN-SEO/LEE, SEUNG-GOO/MHEEN, TAE-ICK/SUNG, MOON-HEE
|
|
|
Abstract
|
|
|
|
A thermophilic bacterium producing D-stereospecific dipeptidase was isolated from Korean soil samples. The enzyme hydrolyzed the peptide bond between D-alanyl-Dalanine (D-Ala-D-Ala). The isolated bacterial strain was rod shaped, gram-positive, motile, and formed an endospore. Morphological and physiological characteristics suggested this microorganism a thermophilic Bacillus species, and was named as Bacillus sp. BCS-1. The production of D-stereospecific dipeptidase was growth-associated and optimal at 55℃. The enzyme was applied for the synthesis of D-amino acidcontaining peptide, Ν-benzyloxycarbonyl-L-aspartyl-D-alanine benzyl ester (Ζ-Ｌ-Asp-D-AlaOBzl), as a model reaction. A thermodynamically controlled synthesis of Ｚ-Ｌ-Asp-Ｄ-AlaOBzl was achieved in an organic solvent.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|